Turning recombinant DNA into a GMO felt like the pinnacle of Genetic Technology. I focused on inserting a pest-resistance gene into a crop plant. After cloning the gene into a Ti plasmid, I used Agrobacterium tumefaciens to infect plant cells. Sketching the bacterium transferring T-DNA into the plant genome helped me visualize how tumor-inducing plasmids become delivery tools.
Regenerating whole plants from transformed cells in selective media—first as callus tissue, then shoots and roots—showed me tissue culture isn’t just jargon. I kept a photo journal of each stage to remember how hormones like auxin and cytokinin direct growth.
Sample SPM Question
“Outline the steps used to produce a transgenic plant using Agrobacterium tumefaciens.”
• Insert foreign gene into Ti plasmid; transform Agrobacterium.
• Infect plant explants; culture on selective medium to regenerate whole transgenic plants.
No comments:
Post a Comment