Gene Therapy & DNA Profiling—Real-World Genetic Technology

 Genetic Technology isn’t only for microbes and crops—it’s revolutionizing medicine. I traced how gene therapy seeks to replace a faulty gene in human cells, using viral vectors like adenovirus or lentivirus. Drawing a stepwise diagram—vector design, patient cell extraction, in-vitro transduction, and reinfusion—clarified why safety and targeting are critical.

I also reviewed DNA profiling, which uses PCR and gel electrophoresis on microsatellite regions. Mapping how variation in short tandem repeats (STRs) produces unique banding patterns helped me understand forensic and paternity testing.

Sample SPM Question
“Explain how DNA profiling can be used in forensic investigations.”
• PCR amplifies STR regions; gel electrophoresis separates alleles by size.
• Compare banding patterns between crime-scene samples and suspects to establish identity.

By writing in my own voice—sketching diagrams, recounting lab trials, and tackling real-life SPM questions—I transformed Genetic Technology from abstract theory into tangible skills. I hope these posts help you see how each KSSM concept fits into hands-on practice and exam success.